Services Test Online

POLYAH - Recognition of 3'-end cleavage and polyadenilation region of human mRNA precursors

Method description:

Algorithm predicts potential position of poly-A region by linear discriminant functions combining characteristics describing various contextual features of these sites. The default LDF threshold in the server is equal 0.


The accuracy has been estimated for the set of 131 poly-A regions and 1466 non-poly-A regions of human genes, having AATAAA sequence. For 86% accuracy poly-A region prediction the algorithm has 8% false predictions (Sp=50%; C=0.62). For example, with threshold 0.7 it predicts 8 of 9 poly-A sites of AD2 genome (35937 bp.) and overpredict 4 false (Compare with method of poly-A site prediction (CABIOS 1994,10,597-603), which for 8 true predicted sites gives 968 false positive sites).

POLYAH output:
First line - name of your sequence; 2nd line - Length of your sequence
Next lines - positions of predicted sites and their 'weights', Position shows the first nucleotide of the AATAAA consensus in the predicted region

For example:

 HSG11C4A     1741 bp    DNA             PRI       21-FEB
 Length of sequence-      1741
     1 potential polyA site was predicted
 Pos.:    988 LDF-  4.06